Downstream Processing

Downstream Processing思维导图，包括：Cell disruption、Clarification、Concentration、normal Purification、ultra Purification、defination。

Downstream Processing

• Cell disruption
  • for the metabolite contained in inclusion body
  • Non-mechanical
    • small scale
    • drying
      • freeze
      • vaccum
    • osmotic shock
    • temperature shock
    • chemolysis
      • autolysis
        • use bacteria own enzymes
      • chemicals
        • EDTA, triton X-100
      • enzymes
        • hydrolyses
        • b-glucanases
      • antibodies
        • peniciline
        • cycloserine
  • Mechanical
    • large scale
    • ultrasonic disrupters
    • homogenisers
      • pressure cells
    • bead mills
• Clarification
  • remove cellular debris
  • Centrifugation
    • Tubular centrifuge
    • Disk-stack centrifuge
  • Filtraction
    • Batch filtration
      • plate filters
    • Continuous filtration
      • rotating drum vaccum filters
• Concentration
  • Evaporation
  • Precipitation
  • Ultrafiltration
    • using membranes
• normal Purification
  • purify bulk product
    • do not requires great purity
  • liquid extraction
    • for small molecules
      • organic solvent
    • for complex molecules
      • aqueous two phase
  • Crystalization
    • supersaturation method
      • cooling
      • evaporation
    • seperated by filtration/cetrofugation
• ultra Purification
  • Chromatography
    • types
      • adsorption
      • affinity
      • gel filtration
      • high performance liquid
      • hydrophobic
      • metal chelate
• defination
  • concentrate and purify the culture broth
    • cost
      • 40%-90%
    • culture broth contain 95% water